Treating Glaucoma With Human Stem Cells: Examining The Potential

by admin | Jun 18, 2021 | Stem Cell Research, Glaucoma, Stem Cell Therapy

Characterized by vision loss caused by progressive damage to the optical nerve, glaucoma continues to be the second leading cause of blindness worldwide. Although painless, the glaucoma-induced cupping, thinning, and structural damage caused to various parts of the eye causes vision loss that starts in the periphery and gradually travels inward, eventually resulting in a total loss of vision.

Current treatments for glaucoma are mainly pharmacologic, laser-based, and surgical procedures that reduce the eye’s intraocular pressure (IOP), the most treatable risk factor of glaucoma.  While these treatments have been demonstrated to be effective in treating the symptoms associated with glaucoma, they are not able to restore vision that has already been lost as a result of glaucoma.

The purpose of Chamiling et al.’s review is to evaluate the current developments surrounding the use and effectiveness of stem cell therapy, not only to treat the symptoms of glaucoma and other optic neuropathic conditions but also to explore the potential of restoring vision loss resulting from these conditions.

According to the authors, while most glaucoma-based therapies center around controlling IOP, they fail to address the main contributing factors associated with glaucoma-associated vision loss – which include axonal damage and loss of retinal ganglion cells (RGCs), the neurons that make up the optical nerve and that are responsible for transmitting visual images from the eye to the brain.

In their natural state, RGCs are what’s considered postmitotic; in other words, they are cells that do not regenerate. This means that any vision loss sustained as a result of the loss of these RGCs is permanent and unable to be reversed. Adding to the severity of early glaucoma is the fact that significant damage to, and loss of, RGCs typically occur before the first signs of developing visual issues are detected.

However, with the recent advancements in cell-based therapies, and considering the field’s rapid-developing understanding of ocular regeneration, there is hope that science will soon be able to advance options that not only treat glaucoma but also restore vision lost as a result of the condition. As such, Chamiling et al. focus this review primarily on the use of stem cell-derived RGCs for drug discovery and transplantation-based therapy in four specific areas: control of intraocular pressure; using pluripotent stem cells as a source of RGCs; stem cell-derived RGCs for transplantation and vision restoration; and using stem cell as a source for neurotrophic factors (NTF).

Through their review of the literature and, in part, a summary of advanced discussions held at the 2015 Ocular Research Symposia Foundation’s “Sight Restoration Through Stem Cell Therapy” meeting, the authors conclude that advancements in our understanding of stem cells combined with key advancements made in the field of ocular biology have resulted in the ability to differentiate human stem cells into a number of different ocular cell types.

While much of the research and trials examined has involved animal models of study, the progression of these efforts has led to a number of human trials exploring the differentiation of stem cells into retinal pigment epithelial cells. In addition, and more specifically related to glaucoma, recent studies demonstrate significant potential for the differentiation of stem cells into trabecular meshwork (TM) and RGCs as well as the opportunity to be used as a way to secrete NTFs.

As a result of this review, Chamling et al. call for continued study into the potential of human stem cells for the treatment of glaucoma while also concluding that the rapid advancement in stem cell technology continues to provide the pathway to further understanding of stem-cell applications in this field and to offer new hope for using cell-based therapies as a way to restore vision lost as a result of glaucoma or other optic nerve conditions.

Source:  (2016, April 1). The Potential of Human Stem Cells for the Study … – PubMed – NIH, from https://pubmed.ncbi.nlm.nih.gov/27116666/

Studying the Potential of Human Stem Cells for Treating Glaucoma

by admin | Apr 23, 2021 | Glaucoma, Stem Cell Research, Stem Cell Therapy

Glaucoma is a group of eye conditions that damage the optic nerve and lead to progressive, irreversible loss of vision. With over 80 million people affected by the condition, glaucoma is the second-leading cause of blindness, behind only age-related macular degeneration.

Although there are several different risk factors, the most understood and treatable risk factor for glaucoma involves controlling the eye’s intraocular pressure, or IOP. When left unaddressed, glaucoma progressively leads to vision loss resulting from damage to axons and associated retinal ganglion cells (RGCs) responsible for transmitting visual information from the eye to the brain. While current treatments for glaucoma are primarily pharmacologic, laser-based, and surgical procedures designed to lower and/or control IOP, they are unable to reverse or restore vision lost as a result of previous damage to the affected axons, RGCs, and the collective optic nerve.

Chanling, Slush, and Zack’s article aim to assess current literature and developments exploring the potential of using human stem cells to further study, and potentially treat, glaucoma and other conditions affecting the optic nerve. For the purposes of this review, the authors divide their discussion into four key areas: stem cell-derived trabecular meshwork cells to control IOP; stem cells as a source of RGCs; stem cell-derived RGCs for transplantation and vision restoration; and stem cells as a source for neurotrophic factors.

Stem Cell-Derived Trabecular Meshwork Cells to Control IOP

Insufficient drainage of the eye’s aqueous humor results in increasing IOP. Current medication for the treatment of this condition reduces aqueous production and or increases aqueous outflow through the trabecular meshwork (TM). Since the TM is a known source of stem cells, researchers hypothesize that these cells and specifically mesenchymal stem cells (MSCs) could be used to repair IOP function and potentially restore vision lost as a result of this condition. 

Recent studies have shown promising results, leading researchers to believe that there is a strong possibility of using stem cell-derived TM cells to preserve optic nerve function and reduce IOP.

Stem cells as a Source of RCGs

In addition to assisting with regulating IOP, researchers believe that stem cells may also be able to preserve, and even restore, RCG function – which is ultimately responsible for vision loss caused by glaucoma.

Specifically, the authors point to a number of animal studies that have demonstrated positive responses in a number of signaling pathways and neuroprotective compounds responsible for promoting RNC function and survival. The authors also point out that, while these studies are promising, none have made it to the clinic.

Stem Cell-Derived RGCs for Transplantation and Vision Restoration

While still a relatively new concept, there has been tremendous progress made in the ability to transplant RCEs and photoreceptor cells in the eye. Coupled with the observed differentiation of RGCs, researchers believe the ability to successfully transplant RGCs, with the intent of restoring glaucoma-related vision loss, is not far off. 

The authors note that, while these findings are promising, there is still much work and additional research to be completed in this area and that the process of transplanting RCGs is much more complicated than the process used for transplanting retinal pigment epithelial cells (RPEs) and photoreceptors cells.

Stem Cells as a Source for Neurotrophic Factors (NTFs)

Research suggests that deprivation of the NTF required for maintenance and survival of neurons is a leading factor in the progression of glaucoma. As a result, additional research has reported that supplementing additional BDNF and other NTFs, through the use of stem cells appears to support the health and survival of RGC. The authors point out that, while promising, the process required during this procedure is challenging, primarily because it requires the blood-retinal barrier to be circumvented.

The authors of this review conclude that, as a result of the rapidly advancing pace of ocular stem cell research and related ongoing advancements in stem cell technology, there are ongoing opportunities to better understand and improve upon the current glaucoma-related biology and to develop pharmacological models that include cell-based therapies in the effort to restore vision to those affected by glaucoma.

Source: (n.d.). The Potential of Human Stem Cells for the Study and … – IOVS  from https://iovs.arvojournals.org/article.aspx?articleid=2518375